Fig. 3

Characterization of chromatin differentially accessible regions in productively HBV-infected PHHs. a) Chromosome localization of the 1804 common DARs identified at 72 h p.i.. The color scale represents the number of DARs localized in each 10 MBps genomic regions. Three different hotspots were identified on chromosomes 1 (35 DARs), 17 (26 DARs), 19 (36 DARs). b) Motif Enrichment Analysis (MEA) of DNA-binding transcription factors (TFs) associated with the genomic sites showing differential accessibility after HBV infection (1804 common DARs). Names are indicated for the top significant TFs. c) Pathway enrichment analysis on the 1537 Differentially Accessible Genes (DAGs) potentially affected upon HBV infection using the ShinyGO 0.76 tool. Top significant KEGG pathways (Release 86.1; yellow bars) and Curated Molecular Signature Database (MSigDB) modules (Release 86.1; blue bars) enriched at 72 h p.i are sorted by –Log (FDR) values. Fold enrichment ranks the relative number of genes included in each enriched pathway (see Table S4). d) CPM distribution for the 85 DARs, corresponding to 81 DAGs that are enriched in the MSigDB, NUYTTEN EZH2 targets UP gene set in MOCK and HBV-infected PHHs (Table S5). e) CPM distribution for 216 regulatory regions (−5000/+ 5000 bp from TSS) in 214 genes showing EZH2 recruitment in vivo by ChIP-Seq in HepG2-NTCP cells (Table S6). ** = p < 0,001