Fig. 1

ATAC-seq analysis of chromatin accessibility changes in HBV-infected PHHs. a ATAC-seq peaks detected at 2 h and 72 h post infection (p.i.) in MOCK (white Box Plots) and HBV-infected (grey Box Plots) primary human hepatocytes (PHHs). Dots represent the total number of peaks detected in each replicate from donor 1 (S1). Triangles represent the total number of peaks detected in each replicate from donor 2 (S2). Sequencing reads alignment to the reference human genome hg19 and peaks calling are detailed in Supplementary Information. b Cumulative percentage of ATAC-seq peaks in MOCK and HBV-infected cells at 2 h and 72 h p.i. as a function of their absolute genomic distance in kilobases (kb) to the closest transcription starting site (TSS). Sequencing reads alignment to the reference human genome hg19 and peaks calling are detailed in Supplementary Information. c. Number of Differential Accessible Regions (DARs) (see the Supplementary Information for details) identified in HBV-infected PHHs from donor 1 (S1) and donor 2 (S2) at 2 h (n = 4129; 4364) and 72 h p.i. (n = 8873; 6256). d Heatmaps of CPM z-scores (see the Supplementary Information for details) of the DARs identified at 72 h in the R1 and R2 replicates from S1 (left panel) and S2 (right panel) donors in HBV (red) and MOCK (blue) conditions. CPM z-scores values are represented with a color scale from blue (negative) to red (positive)