Fig. 5

NKILA negatively regulate IκB phosphorylation and NF-κB activation. a Western blotting showing total and phosphorylated IκBα in A549 and H226 cells. Left panel was representative images and right panel was statistical column diagram. b Western blot for nuclear and cytoplasm p65 in A549 and H226 cells. GAPDH and Histone 3 (H3) is the loading control for cytoplasm and nuclear, respectively. Left panel was representative images and right panel was statistical column diagram. c RIP-derived RNA was measured by qRT-PCR. The levels of qRT-PCR products were expressed as a percentage of input RNA. Data are expressed as means ± SEM. Two-tailed Student’s t-test was used. *p < 0.05, **p < 0.01, ***p < 0.001