Fig. 2

In vitro antitumor activity of Pt@PCN-Cu. (A-C) Cell viability of PANC-1, SW1990 and CFPAC-1 cells following various treatments, assessed using the MTT assay. (D) Representative CLSM images of PANC-1 cells after different treatments. Cells were stained with Calcein-AM and PI. Scale bar: 40 μm. (E) Flow cytometry (FCM) analysis showing the apoptotic rate of PANC-1 cells subjected to different treatments, quantified via APC-Annexin V/PI staining. (F) Representative images of colony formation assays in PANC-1 cells after treatment with different drugs. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA, with **P < 0.01 and ***P < 0.001