Fig. 8
Mn-ER-Cy inhibited tumor growth and metastasis in vivo. (a) Images of proximal tumors and (b) tumor volume curves on day 21 after various treatments (n = 5, mean ± s.d.). (c) Tumor weights and (d) body weights during the treatment period (n = 5, mean ± s.d.). (e-h) Tumor growth curves for individual tumors in mice across various groups. Images of (i) distant tumors and (j and k) tumor volume and weights on day 21 after various treatments (n = 5, mean ± s.d.). (l) Survival analysis of mice in each group during the 45-day observation period after treatment (n = 10). (m) Representative pictures of mouse lung metastasis on day 21. (n) Representative H&E images depicting lung metastasis nodules in each group. Black arrows indicate lung metastasis nodules. The experimental groups were categorized as follows: (I) PBS, (II) 808 nm laser (0.5 W/cm2, 5 min), (III) Mn-ER-Cy, and (IV) Mn-ER-Cy with 808 nm laser. Statistical analyses were conducted using one-way ANOVA followed by Tukey’s test. *p < 0.05, **p < 0.01, ***p < 0.001