Fig. 6
Tumor-targeting accumulation and NIR/PAI dual-modality imaging. (a) NIR fluorescence imaging of Mn-ER-Cy at different concentrations. (b) Semiquantitative analysis of fluorescence intensities from (a). (c) NIR fluorescence imaging analysis of Mn-ER-Cy accumulation in tumor regions at various time intervals. Red circles represent tumor regions. (d) Semiquantitative analysis of fluorescence intensities in tumor tissues from (c). (e) Ex vivo NIR fluorescence imaging of different organs and tissues (liver, heart, spleen, lungs, kidneys, muscle, and tumor) collected from mice 24 h after Mn-ER-Cy injection. (f) Semiquantitative analysis of fluorescence intensities in vital organs and tissues (n = 3, mean ± s.d.). (g) In vitro PAI of Mn-ER-Cy at varying concentrations under 808 nm laser irradiation. Left, axial slice; right, 3D view. (h) Linear relationship between Mn-ER-Cy concentrations and PAI signal intensities. (i) PAI analysis of Mn-ER-Cy accumulation in tumor regions at various intervals. Red circles represent tumor regions. (j) Semiquantitative analysis of PAI signal intensities in tumor tissues from (i), with PAI signal intensities at 0 h serving as the baseline (n = 3, mean ± s.d.)