Fig. 4

ANXA1 interacts with GOT1 and regulates its protein expression. A: Silver staining image before mass spectrometry analysis (IP-ANXA1). B: Enrichment analysis of potential proteins associated with ANXA1 (KEGG and GO). C: Venn diagram based on public database MsigDB, Amino acid metabolism, BIOGRID, and mass spectrometry analysis intersection. D: CO-IP detection of the interaction between ANXA1 and GOT2 in HUCCT1 cells. E: CO-IP detecting the interaction between ANXA1 and GOT1 in HUCCT1 and HCCC9810 cells endogenously. F: CO-IP detecting the interaction between ANXA1 and GOT1 in 293T cells exogenously. G: Immunofluorescence detecting the co-localization of ANXA1 and GOT1 in HUCCT1 and HCCC9810 cells and fluorescence intensity statistics. H: Immunofluorescence detecting the co-localization of ANXA1 and GOT1 in typical ICC tissues. I: Representative immunohistochemical (IHC) staining of ANXA1 and GOT1 in tumor tissues. J: A contingency table was constructed based on IHC scores to assess the expression distribution of ANXA1 and GOT1 in 48 cholangiocarcinoma tissue samples(chi-square test). K: Correlation analysis of ANXA1 and GOT1 expression in intrahepatic cholangiocarcinoma (ICC) tissues based on immunoreactive scores (IRS)(Spearman’s correlation). L: Immunofluorescence detecting the expression of GOT1 in HUCCT1 cells with ANXA1 knockdown. M: Immunohistochemical staining to detect the protein expression level of GOT1 in subcutaneous tumor tissues of HUCCT1 cells with ANXA1 knockdown