Fig. 2

ANXA1 is highly expressed in ICC tissues and cells and is associated with poor prognosis. A: mRNA expression levels of ANXA1 in 40 pairs of ICC tumor and normal tissues(n = 40). B: Protein levels of ANXA1 in 12 pairs of ICC tumor and normal tissues. C-E: mRNA and protein expression of ANXA1 in ICC cell lines and normal biliary epithelial cell lines(n = 3). F-G: Representative immunohistochemical images of ANXA1 in 78 pairs of ICC tissues and corresponding normal tissues (scale bar 250 μm) and statistical graph of immunohistochemical scoring (n = 78, IHC score 0–12). H-I: Kaplan-Meier analysis of overall and disease-free survival in low and high ANXA1 expression groups. Data are representative of three (A, C, E) independent experiments. Unpaired two-tailed Student’s t-tests (A, G); One-way ANOVA with Dunnett’s test (C, E); Log-rank (Mantel–Cox) test (H, I). **, p < 0.01; ***, p < 0.001. Data are mean ± SD