Fig. 6

Epigenetic modulation and functional regulation by MS-275 in DMG cells. A CUT&Tag-seq profiles from TT150630 cells showing sites where treatment with MS-275 (1μM) for 2 days resulted in increased (top) or decreased (bottom) H3K27ac signals at the gene promoter (p < 0.05, |LFC|> 1.5) (n = 2). B-C Average H3K27ac signal corresponding to (A). D Volcano plots showing differentially expressed genes from H3K27ac CUT&Tag signals of TT150630 with or without MS-275 (1 μM) for 2 days. E Canonical pathway (MSigDB) enrichment analysis of 996 DEGs in MS-275-treated DMG cells compared with the DMSO-treated cells. F Integrated Genomics Viewer (IGV) screenshot showing results of H3K27ac CUT&Tag-seq in peaks at the genomic regions of HLA-E