Fig. 1

The inhibitory effects of MS-275 on DMG cells in vitro and in vivo. A-B The inhibitory effects of MS-275 on TT190326 and TT150630 cells were demonstrated via CCK-8 assays. The cells were treated with various concentrations of MS-275 (0.01–100 µM) for 24 and 48 h. C-D Representative immunofluorescence image of EdU-stained TT150630 and TT190326 cells after treatment with 1 μM MS-275 or vehicle for 2 days (left). Average percentage of EdU-positive TT150630 or TT190326 cells after treatment with DMSO or MS-275 (right). Scale bars, 50 μm. Statistical significance was assessed via Student’s t test, with *p < 0.05. E–F Distribution (left) and quantitation (right) of the cell cycle in TT150630 or TT190326 cells treated with DMSO or 1 μM MS-275 for 2 days. Statistical significance was assessed via Student’s t test, with **p < 0.01, ***p < 0.001, and ****p < 0.0001. G-H Flow cytometry analysis of apoptotic TT150630 or TT190326 cells after treatment with DMSO or 1 μM MS-275 for 2 days (left). The percentages of Annexin V-positive and PI-negative (early apoptotic) cells were quantified with FlowJo software (right). Statistical significance was assessed via Student’s t test, with **p < 0.01 and ***p < 0.001. I Representative bioluminescence images (BLIs) of mice xenografted with TT150630-luciferase cells and treated with or without MS-275 (n = 5/group). J Quantification of the BLI signal in (I). Statistical significance was assessed via Student’s t test, with *p < 0.05 and **p < 0.01. K Kaplan‒Meier survival curves for mice xenografted with TT150630-luciferase cells and treated with or without MS-275 (n = 5/group). P values were calculated via the log-rank test