Fig. 5

Validation of tumorigenic potential of lung organoids. A) Images of mice used to examine the tumorigenic characteristics of organoids. KP/healthy and KPC/KPC-LM organoids were simultaneously transplanted. Red circles indicate the formed tumors. B) Schematic for lentivirus transduction to obtain iRFP-Luciferase positive cells. C) In vitro evaluation of luciferase activity for serially diluted transduced MLT3M2-iRFP⁺-Luc.⁺ cells using IVIS spectrum. D) Visual changes in BLI signal intensity in athymic nude mice following 6 weeks of post-transplantation, ex vivo image of resected lung, and line graph illustrating increase in average radiance overtime. D) Representative IF staining images of TTF-1 and CK7 expression individually and merged with DAPI (blue). TTF-1; Thyroid transcription factor-1, CK7; Cytokeratin 7