Fig. 2

Establishment of organoids from murine healthy lung, LUAD, and LM tissues. Murine lung and liver metastatic tissues were collected to generate organoids based on relevant mutations. A) Schematic for organoid generation from primary tissues. B) One-week expansion of the KP and KPC organoid models (5X) with scale bars; 200 μm. and their morphology (10X) with scale bars; 50 μm. C) Comparison of average organoid diameter between models (n = 15 per group). ****p ≤ 0.0001. D) Representative images from healthy organoids. 2 biological replicants of KP organoids (MLT5 and MLT 18), 2 biological replicates of KPC organoids (MLT3 and MLT9) and metastatic liver organoid (MLT3L)