Fig. 2

DNMT3B expression in human MM cell lines and patient samples. A) DNMT3B mRNA levels in 40 different HMCLs using our own RNA-Seq data. B) DNMT3B mRNA expression in a selected panel of HMCLs using qRT-PCR. ABL was used as a reference gene. The mean expression ± SD for three independent experiments is shown. C) DNMT3B protein expression determined in a selected panel of HMCLs via western blot. Tubulin was used as loading control. Left: blots of one experiment representative of three are shown, right: quantification of the DNMT3B levels relative to tubulin as measured by Image Studio for the 3 independent experiments. D) The DNMT3B levels in PCs obtained from one monoclonal gammopathy of undetermined significance (MGUS), one smoldering myeloma (SMM), 3 newly diagnosed (ND) and 3 relapsed (Relapse) MM patients were determined using qRT-PCR. ABL was used as reference gene.