Fig. 5

p38-ROS regulates NETs formation through c-FOS. A Detection of protein expression levels of PAD4, p38, and p-p38 in neutrophils after stimulation with CM from various cell lines. B IF detection of ROS expression levels in neutrophils after stimulation with CM from various cell lines. C After simultaneously stimulating neutrophils with MDA-MB-231 or HCC1937 CM and inhibiting ROS (Acetylcysteine), inhibiting the p38 pathway (SB203580), activating the p38 pathway (Metformin HCl), or through combined interventions, detection of protein expression levels of PAD4 and c-FOS in neutrophils. D, E After simultaneously stimulating neutrophils with MDA-MB-231 or HCC1937 CM and inhibiting ROS (Acetylcysteine), inhibiting the p38 pathway (SB203580), activating the p38 pathway (Metformin HCl), or through combined interventions, IF detection of expression levels of c-FOS and SYTOX GREEN in neutrophils. Data are presented as means from three independent experiments ± S.D. **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, nonsignificant