Fig. 4
From: RASON promotes KRASG12C-driven tumor progression and immune evasion in non-small cell lung cancer
RASON knockout inhibits lung cancer progression in vivo and restores macrophage infiltration. A, Effects of RASON KO on KRAS signaling pathways in MEFG12C, H358, and LLC cell lines. B, Effects of RASON KO on in vitro proliferation of MEFG12C, H358, and LLC cell lines. C-E, Effects of RASON KO on H358-derived xenograft tumor growth in vivo. (C) Representative images of H358 xenograft tumors in nude mice. Scale bar, 1 cm. (D, E) Quantitative analysis of tumor weights and volumes (n = 7 per group). F-G, Effects of RASON KO on LLC lung colonization formation in vivo. F, Representative H&E staining images of lung sections from LLC lung colonization models in C57B6/J mice. Scale bar, 2 mm. G, Quantitative analysis of tumor area (n = 6). H, Effects of RASON KO on CD47 expression in H358 and LLC cell lines. I, IHC staining showing CD11b and CD47 expression levels in LLC-derived lung tumor tissues. Scale bar, 50 μm. Data are shown as mean ± S.D. and analyzed by Student’s t-test (D, E, G) or one-way ANOVA (B, E). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001