Fig. 6

TRAF7 depletion inhibits glioma proliferation and induces senescence via KLF4. A Kaplan–Meier survival using TCGA on KLF4. B The overexpression of KLF4 in Hs683 and U251 cells. C The expression of TRAF7 and KLF4 in the Hs683 and U251 glioma cell lines. D Co-immunoprecipitation (Co-IP) assays were performed using lysates from U251 cells transfected with Flag-tagged KLF4 and HA-tagged TRAF7. E–F Colony formation assays were performed in the glioma cell lines with or without KLF4 overexpression. G-H SA-β-gal staining was performed in the glioma cell lines (scale bar = 50 µm). I-J The rescue colony formation assay showed that the inhibitory effects of TRAF7 knockdown on cell proliferation could be rescued by additional KLF4 overexpression in cells. K-L The rescue SA-β-gal staining assay showed that the promotion effects of TRAF7 knockdown on cell senescence could be rescued by additional KLF4 overexpression in cells (scale bar = 50 µm). *p < 0.05, **p < 0.01, and ***p < 0.001