Fig. 5

Combination of Lomustine (CCNU) and sh-TRAF7 promotes glioma senescence and G0/G1 arrest. A IC50 of lomustine (CCNU). B Cell viability of TRAF7 deficient cell lines treated with low concentrations of lomustine (1 μM and 10 μM) for 12 h. C Cell viability of Hs683 and U251 cells treated with lomustine (50 μM). D SA-β-gal staining after CCNU treatment of the control group and sh-TRAF7 group in the cells. E–F The mRNA expression and protein expression of G0/G1 arrest related genes after CCNU treatment. G-H mRNA expression and protein expression of senescence related genes after CCNU treatment in the cells