Fig. 4
TRAF7 loss sensitizes glioma to senescence and G0/G1 arrest by RNA sequencing. A Volcano plot of differentially expressed genes between the control group and siTRAF7 group in the Hs683 and U251 cells. B GSEA revealed the enrichment of differentially expressed genes. C The pathway enrichment analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG). D Heatmap of the differentially expressed (|log2FC|> 2 and p-value < 0.05) genes via RNA-seq. E The Hs683 and U251 cells with TRAF7 knockdown induced senescence. F Knockdown of TRAF7 induced G0/G1 arrest of Hs683 and U251 cells. G The heatmap of senescence and cell-cycle related genes. H-I mRNA expression of G0/G1 arrest related genes. J Protein expression of G0/G1 arrest related genes in Hs683 and U251 cells. K-L mRNA expression of senescence related genes in Hs683 and U251 cells. M–N The protein expression of G0/G1 arrest and senescence related genes