Fig. 6

NAT10-mediated ac4C modification promotes migration, invasion, and metastasis via ITGB5. (A-B) Representative image and quantification of Transwell invasion in the NAT10 ^G641E mutant of NAT10-KD PANC-1 (A) or MIA PaCa-2 (B) cells. Scale bar: 200 μm. (C) Representative immunoblotting image of NAT10 and ITGB5 in PANC-1 (left) and MIA PaCa-2 cells (right). (D) Representative image and quantification of Transwell assay results for ITGB5-overexpressing NAT10-KD PANC-1 cells. Scale bar: 200 μm. (E) Representative image (left) and quantification (right) of DRG model in NAT10 G641E mutant and ITGB5-overexpressing PANC-1 cells. Upper scale bar: 200 μm; lower scale bar: 50 μm. (F-G) Representative images (F) and quantification (G) of lung metastasis detected via bioluminescence imaging. (H-I) Representative H&E images (H) and the area of the tumor (I) of indicated groups. Scale bar: 200 μm. *p < 0.05, **p < 0.01, ***p < 0.001. (A-B), (D-E), (G), and (I), One-way ANOVA