Fig. 4

Label-free quantification analysis. (A) Heatmap showing the hierarchical clustering of the different EV preparations as a function of the differentially expressed proteins. CTC-derived EVs were separated from CRC cell-derived EVs, and EV samples were separated from the three CTC lines. (B) Principal component analysis showing clustering of the CTC-derived EV samples. CTC-MCC-41.4 and 41.5G clustered together in contrast to CTC-MCC-41, this in relation to their clonal status. (C) Correlation matrix showing a greater correlation between CTC lines