Fig. 2

Cellular component analysis of the EV proteomic cargo based on present-absent analysis. A) to E) Comparison of the EV proteins identified with those listed in Vesiclepedia and those identified by Hoshino et al. (2020). F) ANXA5 was not detected in EV preparations from the three CTC lines or the two colon cancer cell lines, and CD9 was absent only in SW480 and SW620 cell-derived EVs. G) Cellular component enrichment in the indicated EV samples according to the Vesiclepedia data for “Cytoplasm” and “Exosomes”, revealing significantly greater enrichment of “Exosome”-associated proteins in EVs from CTC-MCC-41.4 and CTC-MCC-41.5G cells (derived after therapy failure) than in those from the other colon cancer cell lines