Fig. 8

USP10 interacts with XAB2. A Distinct bands stained with silver and identified by MS. B Lysates from SW480 and HCT116 cells immunoprecipitated with the IgG control, anti-XAB2 antibody, or anti-USP10 antibody followed by immunoblotting with USP10 and XAB2 antibodies. C Interaction between Flag-USP10 and Myc-XAB2 confirmed by Co-IP in HEK293T cells. D Increase in nuclear expression of USP10 in CRC cells verified by IF after treatment with oxaliplatin (7.5 µM) for 24 h, which can be inhibited by the ATM inhibitor Ku55933 (20mM). E Increase in nuclear expression of USP10 in CRC cells verified by western blot analysis after treatment with oxaliplatin (7.5 µM) for 24 h. F Schematic representation of Flag-tagged full-length (FL) USP10 with its various deletion mutants. G Co-IP confirming the interaction between Myc-XAB2 and Flag-tagged FL USP10 or its indicated mutants in HEK293T cells