Fig. 3

Coordinated effect of the CD28 hinge and DAP10 EC domain on the surface expression of NK-CARs. A Schematic diagram of CAR constructs with the indicated combinations of H and DAP10 domains with the 2B4 CYP domain. B Representative flow cytometry analysis showing the MFI of GFP (left panel) and surface CAR (right panel) expression on the CAR-expressing NKL cells. C Summary graph showing the MFI of surface CAR expression in CAR-transduced NKL cells. D Comparison of the lysis of REH, Raji, or Ramos cells by the indicated CAR-expressing NKL cells at the indicated E:T ratio. Values represent the means ± SD. Data were analyzed using the one-way (C) or two-way ANOVA (D) with Dunnett’s multiple comparison test. ns, not significant; *P < 0.05; ***P < 0.001; ****P < 0.0001 relative to EV NKL cells (C) or NK-CAR6 NKL cells (D). Data are representative of three independent experiments