Fig. 6
Knockout of AMPK catalytic subunits impairs tumor growth in vivo
(A) Athymic nude mice were intracranially injected with 1 × 105 G55T2 wildtype (wt) or AMPK catalytic subunits double knockout (DKO) cells (n = 10 mice per group). MRI measurements were performed on day 11, 18 and 25 after tumor cell injection. Images of 3 mice per group are shown exemplarily. (B) Tumor volumes were calculated based on MRI measurements on day 11 and 18 using the ITK Snap software. (C) Survival of mice (n = 10 per group) injected with G55T2 wildtype or AMPK DKO cells was analyzed by Kaplan-Meier Plot. Significance was tested using Log-Rank test. (D) Survival of mice (n = 20 per group) injected with LNT-229 wildtype or AMPK DKO cells was analyzed by Kaplan-Meier Plot. Significance was tested using Log-Rank test. Data of two independent experiments were pooled. (E) G55T2 wildtype and AMPK DKO tumors (left panel) were analyzed immunohistochemically with antibody for P-ACC. Scale bar represents 200 μm (10x magnification, bottom panel) or 1 mm (2x magnification, upper panel). LNT-229 wildtype and AMPK DKO tumors (right panel) were also analyzed with antibody for P-ACC