Fig. 5
AMPK catalytic subunits double knockout cells are sensitized to 2-deoxyglucose treatment
(A) CV staining of LNT-229 wildtype and AMPK catalytic subunits double knockout (DKO) cells was performed after treatment with vehicle (DMSO), 2 mM (left panel) or 5 mM (right panel) 2-DG in serum-free medium supplemented with 5 mM glucose for 72 h. Results are shown as absorption at 595 nm and relative to T0, reflecting the respective cell density at the beginning of the experiment. (B) LNT-229 wildtype and AMPK DKO cells were treated with vehicle (DMSO) or 2 mM 2-DG in serum-free DMEM with 2 mM glucose in normoxia or hypoxia (0.1% O2). LDH release assay was used for cell death analysis (n = 4, mean ± SD, n.s. not significant, *p < 0.05, **p < 0.01, Student’s t-test)