Fig. 6

NONO and PKM2-mediated H3T11ph coordinate with H3K27ac on SERPINE1 gene loci. (A) Western blot analysis of the indicated histone H3 modifications in NC and PKM2-silenced MDA-MB-231 cells. Histone H3 served as a loading control. (B) Heatmaps of CUT&Tag data showing that NONO, H3T11ph, and H3K27ac levels at the transcription start site (TSS) significantly decreased (Decreased) or unchanged (Others) in the NC, NONO-KD, or PKM2-KD MDA-MB-231 cells, respectively. (C) Integrative Genomics Viewer (IGV) tracks representing the signals of NONO, H3T11ph, and H3K27ac at SERPINE1 gene loci from CUT&Tag data in MDA-MB-231 cells. (D) ChIP‒qPCR analysis of the enrichment of NONO, H3T11ph, and H3K27ac at SERPINE1 gene loci at the indicated positions as in (C) in MDA-MB-231 cells. IgG served as a negative control. The data are presented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 compared to the corresponding control