Fig. 4

Anti-PD-1 plus anti-TIGIT combination therapy activating systematic anti-tumour immunity. a, b, c Representative flow-cytometry plots (Day 13) showing the tumour-infiltrating CD4 + , CD8 + , CD4 + FoxP3 + (Treg) cells. d Enumeration of CD4 + , CD8 + , T reg cells in tumour. Representative flow-cytometry plots and relative percentage of e) NK cells and f) PD-1, g CTLA-4, h TIGIT expression on CD8 + T cells in tumour. N = 4–5 mice were used per group and per timepoints. Error bars reflect the SD determined from each treatment group. Statistical significances were calculated by two-way ANOVA. * P < 0.05, **P < 0.01, ***P < 0.01, ****P < 0.0001