Fig. 2

ELK3 transcriptionally represses expression of CYFIP2 in TNBCs. A Heat map displaying the expression levels of WAVE complex subunits (CYFIP2, WASF3, BRK1, CYFIP1, NCKAP1, ABI2) identified by RNA-sequencing analysis of MDA-MB-231 control, ELK3KD cells and ELK3KD + ELK3 rescued cell. B–C Quantitative RT-PCR and immunoblot analysis of CYFIP2 in control and ELK3KD TNBC cells that were transfected with a control vector (CV) or an ELK3-expressing vector (ELK3). Control (Cont) = sh control of MDA-MB-231 or Hs578T cells; ELK3KD = ELK3KD of MDA-MB-231 or Hs578T cells. D Luciferase reporter assay showing activity of the CYFIP2 promoter. ELK3KD MDA-MB-231 cells were transfected with the indicated plasmid combinations for 48 h, followed by a luciferase assay. E ChIP-qPCR analysis of ELK3 binding to the CYFIP2 promoter. A Flag-ELK3-expressing plasmid or Flag-control plasmid was transfected into ELK3KD cells for 48 h, and Flag-immunoprecipitates were subjected to qPCR using primers specific for the CYFIP2 promoter region (−1450 to + 50 bp). All data were derived from at least three independent biological experiments. Data are presented as the mean ± standard deviation (SD). NS indicates no statistical significance. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001