Fig. 1

ELK3 expression levels are associated with filopodia protrusion in TNBC cells. A Protrusion of filopodia from MDA-MB-231 and Hs578T cells was compared with that from ELK3KD cells transfected with an empty matching plasmid (control vector; CV) or an ELK3-expressing vector (ELK3). Cells were stained with DAPI and phalloidin. Actin accumulation associated with filopodia formation was visualized using fluorescence microscopy; representative protrusions are indicated by red arrows. Scale bar, 20 µm. B The number of filopodia per cell were quantified and is presented as individual dots. (MDA-MB-231 cells, n = 29, 30, and 30, respectively, Hs578T cells, n = 16, 67, and 35, respectively.) C The length of filopodia are presented in a graph. (MDA-MB-231 cells, n = 29, 30, and 30, respectively, Hs578T cells, n = 14, 17, and 17, respectively.) Data are presented as the standard error of the mean (SEM). Control (Cont) = sh control of MDA-MB-231 or Hs578T cells; ELK3KD = ELK3KD of MDA-MB-231 or Hs578T cells. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001