Fig. 10

Cinobufagin promotes T Cell activation and TAMs Reprogramming. A-D Percentages of Ki67⁺, CD69^+, TNFα⁺, IFNγ⁺, GzmB⁺, and PD1⁺ of total CD8⁺ and CD4⁺ T cells in GL261 tumors identified by flow cytometry. N = 6 mice/group. E-F IF staining of GzmB⁺ and CD206⁺ cells in CT2A tumors. N = 6–7 mice/group. G Percentages of PD-L1⁺ cells among total F4/80⁺ cells in GL261 tumors identified by flow cytometry. H-I The percentages of CD80⁺, CD206⁺, iNOS⁺, CD40⁺, MHC-I⁺, and MHC-II⁺ cells among total F4/80⁺ cells in GL261 tumors identified by flow cytometry. J-K Percentages of monocyte-derived macrophages (MdMs) and brain-resident microglia (BrMs) among F4/80⁺ cells and CD45⁺ cells in GL261 tumors identified by flow cytometry, respectively. L-M Mean fluorescence intensity (MFI) of CD80, CD206, iNOS, CD40, MHC-I, and MHC-II and PD-L1 in MdMs and BrMs identified in GL261 tumors via flow cytometry