Fig. 7
Development of the ALDH1A1/MMP11-related plasma proteome-based prognostic signature. A “High-risk” and “low-risk” samples were selected in the Oli-P study cohort based on PSA-free survival time and MMP11 levels measured by ELISA assay. “Yes” or “no” indicate the occurrence or absence of PSA increase (20% above nadir), correspondingly. B The Kaplan–Meier analysis of the PSA increase in high risk (N = 5) and low risk group (N = 5). C Days to PSA increase in high risk (N = 5) and low risk group (N = 5); Error bars = SD; ***p < 0.001. D MMP11 levels measured by ELISA assay in high risk (N = 5) and low risk group (N = 5); Error bars = SD; **p < 0.01. E Schematic diagram of the proteomics analysis of plasma samples. F A subset of 8 non-immunoglobulin proteins detected by mass spectrometry that significantly correlate with MMP11 ELISA values; *p < 0.05; ***p < 0.001. Color scale indicates log2 of relative protein plasma levels normalized to the median. G Abundances of 8 selected plasma proteins in high-risk (HR; N = 5) vs. low-risk group (LR; N = 5); Error bars = SD; ***p < 0.001; *p < 0.05. H Analysis of the potential correlation between the expression levels of 8 genes coding selected plasma proteins and 5 genes from ALDH1/MMP11 pathway in the SU2C/PCF Dream Team cohort of the patients with metastatic PCa (N = 266); *p < 0.05. I LNCaP cells were treated with ATRA at a concentration of 50 μM or DMSO as a control for 48 h. Relative gene expression was analysed using RNA sequencing analysis as described previously [40]. n ≥ 3; Error bars = SD; **p < 0.01. J The Kaplan–Meier analyses of the association of 8-gene signature expression and clinical outcomes in the PCa gene expression dataset SU2C/PCF Dream Team (n = 81). To evaluate performance of the 8-gene signature for the patients ‘ stratification, the median of log2 transformed and quantile normalized expression values of those 8 genes were calculated for each patient. Obtained median values were used for the stratification of the patients into high and low groups by the most significant cut-off