Fig. 3

RARA- and AR- interplay drives TGFB1 transcription. A qPCR analysis of the relative TGFB1 expression in LNCaP cells upon knockdown of AR, RARA, and RXRA expression alone or in combination. Cells transfected with scrambled siRNA (siScr) were used as a control. n ≥ 3; Error bars = SD; *p < 0.05; **p < 0.01; ***p < 0.001. B Chromatin immunoprecipitation (ChIP)-qPCR analysis in LNCaP cells for assessment of the direct binding of RARA and AR proteins to the putative binding regions in the promoter of the TGFB1 gene. RARA and AR binding sites were taken from the JASPAR CORE database [64]. n ≥ 3; Error bars = SD; *p < 0.05; **p < 0.001; ***p < 0.001