Fig. 2

Two distinct states of tumor associated macrophages (TAMs) in PitNETs. A UMAP of 8,560 monocytes and macrophages colored by assigned cell type. B Dot plot depicting the average expression of cell-type-specific markers across cell clusters. The dot size indicates the proportion of cells expressing each marker gene within the cluster. C Bar plot displaying the proportion of each cell type across Bone Invasive states. D Inferred developmental trajectory of monocytes and macrophages using monocle3, with colors representing psedotime. E Violin plot showing the expression of myeloid-derived suppressor cells (MDSCs) signatures. F Bar plot depicting pathways enriched in genes that exhibit higher expression in monocyte-derived (Mo) macrophages compared to tissue-resident (Rs) macrophages. G UMAP plots showing the expression levels of selected marker genes in CX3CR1 + TREM2 + TAMs. H Density plot visualizing combined gene expression of CX3CR1 + TREM2 + Mo-TAMs markers. I, J Differentially expressed genes (left) and differentially activated pathways (right) between CX3CR1 + TREM2 + Mo-TAMs and CX3CR1 + TREM2 + Rs-TAMs