Fig. 7

Tumor-derived MIF promotes myeloid cell phenotypic transition through the JAK/STAT signaling pathway. A MIF expression results from HNSCC bulk RNA sequencing data from the TCGA database are presented, showing range and mean ± SEM. Statistical analysis was conducted using the Wilcoxon test. B Protein expression levels of MIF in serum from normal mice versus mice with OSCC. C Protein expression levels of MIF in conditioned media from normal oral epithelium and OSCC epithelial cells. D Changes in mRNA expression levels of CD74, PDGFRA, ACTA2, PDGFRB, VIMENTIN, and FAP in THP-1 cells treated with or without MIF. E and F Changes and quantitative bar graphs of protein expression levels of FAP and CD74 in THP-1 cells treated with or without MIF. G A bar chart presents the results of GO and KEGG enrichment analysis of differentially expressed genes in myeloid cells between HNSCC and normal oral mucosa. H Changes of p-STAT3 protein expression levels in THP-1 cells treated with conditioned media from normal oral epithelium and OSCC epithelial cells. I Changes in protein expression levels of p-STAT3, CD74 and FAP in THP-1 cells treated with conditioned media from normal oral epithelium and OSCC epithelial cells, and treated with the MIF inhibitor HY13818. Statistics are shown in mean ± SD (B, C, D, and F) accessed by the unpaired t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant, respectively