Fig. 6

Knockdown of MIF in SCC7 cells results in a decrease in apCAF numbers. A Changes in MIF mRNA expression levels in SCC7 cell lines 24 h after treatment with different siRNAs. B Gross images and quantitative results showing tumor volume changes 14 days after inoculation of SCC7 cell lines treated with siRNA-320 or siRNA-NC. C and D Flow cytometry dot plots and quantitative results showing changes in the number of CD74⁺PDGFRB⁺ cells in tumor tissue from both groups of mice. E and F Flow cytometry dot plots and quantitative results showing changes in the number of FAP⁺ cells in tumor tissue from both groups of mice. Statistics are shown in mean ± SD (F) accessed by the unpaired t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant, respectively