Fig. 2

Mouse oral squamous cell carcinoma cell populations contain PDGFRB⁺CD74⁺ cells. A UMAP dimensionality reduction and visualization results show the entire oral squamous carcinoma cell population is divided into 8 cell clusters. B Violin plots display the expression levels of highly expressed marker genes across all cell clusters among the 8 identified cell clusters. C UMAP dimensionality reduction and visualization results further detail the division of the 8 cell clusters into 19 cell subpopulations. D Feature plots demonstrate the presence of a CAFs cell population co-expressing the genes CD74 and PDGFRB. E Multicolor immunofluorescence staining of oral squamous carcinoma tissue shows cells co-expressing Cd74 and Pdgfrb (indicated by white arrows). F Flow cytometry results of oral squamous carcinoma cells reveal the proportion of CD74⁺PDGFRB⁺ cells within the carcinoma (Q2 quadrant)