Fig. 3

Landscape of CD45⁺ immune cells in response to IPG0521m treatment. A The scheme of the single cell RNA sequencing. B The Uniform Manifold Approximation and Projection (UMAP) of 10 × -based tumor infiltrated CD45⁺ immune cells single-cell data from 6 mice of the IPG0521m administration and control group showing 18 clusters in TIME identified by the integrated analysis, colored by cell cluster. Each single dot corresponds to one single cell colored according to cell cluster. C Gene bubble plot showing the expression of well-recognized marker genes in the major cell types. D Pie plot of cell cluster distribution in the IPG0521m administration group and control group, respectively. E The proportion of cell clusters in tumor infiltrated CD45⁺ immune cells in response to IPG0521m treatment. Data were shown as mean ± SD. **p < 0.01, ***p < 0.001, compare to control