Fig. 2

SNORA37 promotes tumorigenesis and aggressiveness of gastric cancer. a MTT colorimetric assay showing the relative viability of HGC-27 and AGS cells stably transfected with scramble shRNA (sh-Scb) or sh-SNORA37 #1 (n = 5). b and c Representative images (left panel) and quantification (right panel) of soft agar (b) and matrigel invasion (c) assays indicating the growth and invasion of HGC-27 and AGS cells stably transfected with sh-Scb, sh-SNORA37 #1, or sh-SNORA37 #2 (n = 5). d In vivo imaging, growth curve, and weight at the endpoints of xenograft tumors formed by subcutaneous injection of MKN-45 cells stably transfected with empty vector (mock) or SNORA37, or AGS cells stably transfected with sh-Scb or sh-SNORA37 #1 into dorsal flanks of nude mice (n = 5 for each group). e Representative images (upper panel) and quantification (lower panel) of immunohistochemical staining showing the intertumoral expression of CD31 and Ki-67 (brown, arrowheads) within subcutaneous xenograft tumors of nude mice formed by MKN-45 or AGS cells stably transfected with mock, SNORA37, sh-Scb or sh-SNORA37 #1 (n = 5 for each group). f and g Representative images (f), hematoxylin & eosin (HE) staining (g, upper panel), quantification (g, bottom left panel) of lung metastatic colonization (arrowheads) and Kaplan–Meier curves (g, bottom right panel) of nude mice treated with tail vein injection of MKN-45 or AGS cells stably transfected with mock, SNORA37, sh-Scb or sh-SNORA37 #1 (n = 5 for each group). One-way analysis of variance (ANOVA) was used to compare the difference in a-d. Student's t-test compared the difference in d, e and g. Log-rank test was used for survival comparison in g. *P < 0.05, ** P < 0.01, *** P < 0.001. Data are shown as mean ± s.e.m. (error bars) and representative of three independent experiments in a-c