Fig. 1

NAT10 promotes proliferation and inhibits apoptosis of CRC cells in vitro. A. NAT10 expression in various cell lines based on CCLE datasets. B, C. Knockdown or over-expression transfection efficiency of NAT10 in SW620, SW48, DLD1 and CACO2 cells determined using RT-qPCR and WB. D, E. Effects of NAT10 on proliferation were measured using the EdU assays (D) and colony formation assays (E). F. Flow cytometry was used to detect rates of apoptosis (LR + UR) of indicated cells. G. The cell cycle distribution was detected by flow cytometry in NAT10 knockdown or overexpression cells. The data are representative of three independent experiments and presented as the mean ± SD. Comparisons were performed using two-tailed unpaired Student’s t-tests. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001