Fig. 7

Inhibition of GPR137-RAB8A hampers OC progression in vivo. A Nude mice with xenografts derived from SK-OV-3 cells stably expressing GPR137-shRNA (shRNA-GPR137) or control viruses (shRNA-Control). N = 6. B Image showing the size of the tumor xenografts from two groups. C Body weight of mice in (A) was examined on day 3, 6, 9, 12, 15, and 18, after tumor cell infection. D Tumor volume in (A) was measured on day 3, 6, 9, 12, 15, and 18, after tumor cell infection. E Nude mice with in situ ovarian tumor from A2780 cells stably expressing RAB8A-shRNA (shRNA-RAB8A) or control viruses (shRNA-Control). N = 6. F Image showing the size of the tumors from two groups. G Body weight of mice in (E) was examined on day 4, 8, 12, 16, 20, 24, and 28, after tumor cell infection. H Weights of xenografts derived from SK-OV-3 cells stably expressing GPR137-shRNA (shRNA-GPR137) or control viruses (shRNA-Control) on day 18 post inoculation. I Weights of ovarian tumors derived from A2780 cells stably expressing RAB8A-shRNA (shRNA-RAB8A) or control viruses (shRNA-Control) on day 28 post inoculation. J Ki67 staining for tumor tissues derived from A2780 cells stably expressing RAB8A-shRNA (shRNA-RAB8A) or control viruses (shRNA-Control) on day 28 post inoculation. Bar,100 μm. K Statistical analysis of Ki67-index (%) from (J). L Annexin V-mCherry staining of tumor tissues derived from A2780 cells stably expressing RAB8A-shRNA (shRNA-RAB8A) or control viruses (shRNA-Control) on day 28 post inoculation. Nuclei were stained with DAPI. Bar,100 μm. M Statistical analysis of Annexin V-mCherry positive cells (%) from (L). N The IHC staining of GLI1 and PTCH1 in formed in situ ovarian tumors derived from A2780 cells stably expressing RAB8A-shRNA or control viruses (shRNA-Control). Bar,100 μm. O The protein levels of GLI1, PTCH1, and RAB8A in formed in situ ovarian tumor tissues derived from A2780 cells stably expressing RAB8A-shRNA (shRAB8A, +) or control viruses (shRAB8A, -). Bar,100 μm.** p < 0.01; error bar, SD