Fig. 3

GPR137 enhances RAB8A mRNA stability. A IHC score for GPR137 and RAB8A in OC tissues. B The protein expression of RAB8A in SK-OV-3 cells transfected with GPR137 shRNA or scrambled shRNA (Con). C The protein expression of RAB8A in A2780 cells transfected with GPR137 shRNA or scrambled shRNA (Con). D Co-immunoprecipitation of endogenous GPR137 and RAB8A in SK-OV-3 cells. IP: RAB8A; WB: GPR137. IgG was used as a negative control. E Co-immunoprecipitation of endogenous GPR137 and RAB8A in separated membrane fraction from SK-OV-3 cells. IP: RAB8A; WB: GPR137. IgG was used as a negative control and EZRIN was used as loading control for membrane protein. F Co-immunoprecipitation of endogenous GPR137 and RAB8A in A2780 cells. IP: RAB8A; WB: GPR137. IgG was used as a negative control. G Co-immunoprecipitation of endogenous GPR137 and RAB8A in separated membrane fraction from A2780 cells. IP: RAB8A; WB: GPR137. IgG was used as a negative control and EZRIN was used as loading control for membrane protein. H Co-immunoprecipitation of endogenous GPR137 and RAB8A in SK-OV-3 cells. IP: GPR137; WB: RAB8A. IgG was used as a negative control. I The mRNA expression of RAB8A in SK-OV-3 and A2780 cells transfected with GPR137 shRNA or scrambled shRNA (shRNA-Con). J The RAB8A promoter-luciferase (RAB8A-Luc) activities in SK-OV-3 and A2780 cells transfected with GPR137 shRNA or scrambled shRNA (shRNA-Con). K The mRNA levels of RAB8A in SK-OV-3 cells transfected with GPR137 shRNA (shGPR137) or scrambled shRNA (shCon) and cultured with actinomycin D (AcD) for the indicated time periods. L The mRNA levels of RAB8A in A2780 cells transfected with GPR137 shRNA (shGPR137) or scrambled shRNA (shCon) and cultured with actinomycin D (AcD) for the indicated time periods. M qRT-PCR analysis of co-precipitated RAB8A mRNA by GPR137 antibody in the RIP assay in SK-OV-3 cells. IgG was used as a negative control. Protein loading was also determined (Input). *p < 0.05; **p < 0.01; error bar, SD