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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: E. Coli cytotoxic necrotizing factor-1 promotes colorectal carcinogenesis by causing oxidative stress, DNA damage and intestinal permeability alteration

Fig. 4

CNF1 alters 3D Caco-2 spheroids formation and disrupts cellular junctions. (A) Representative images from CLSM examinations (central optical sections and 3D reconstruction images). 3D Caco-2 spheroids were stained with anti-ZO-1 (green) and phalloidin (red) to visualize actin proteins. Nuclei are stained with DAPI (blue). Separate channels and merged images are shown. In the insert of control spheroids a higher magnification of ZO-1 distribution is shown. 3D reconstructions of the entire spheroids are reported on the right. Scale bars, 50 μm. Representative examples of 3 independent experiments are shown. (B) Representative scanning electron microscopy micrographs of Caco-2 spheroids grown in control medium, or in presence of CNF1 and CNF1-C866S toxins, showing their 3D overall spatial architecture comprehensive of the surface features. (C) Representative transmission electron microscopy micrographs of Caco-2 spheroids cultured in control medium, or in presence of CNF1 and CNF1-C866S toxins showing their internal ultrastrucural organization (TJ = tight junction; AJ = adherens junction; * Intercellular spaces)

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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