Fig. 5

ONC213 + venetoclax induced cell death is partially dependent on Mcl-1. (A-C) MV4-11, THP-1, and MV4-11/VEN + AZA-R cells were treated with VEN and ONC, alone or in combination, for up to 48 h. Representative western blots of whole cell lysates probed with the indicated antibodies are shown. The fold changes for the densitometry measurements, normalized to β-actin and then compared to vehicle control, are indicated below the corresponding blots. (D) MV4-11 cells and THP-1 cells (cultured in glucose or galactose containing media) were treated with VEN, ONC, or ISRIB, alone or in combination, for 8–16 h. Representative western blots of whole cell lysates probed with the indicated antibodies are shown. The fold changes for the densitometry measurements, normalized to β-actin and then compared to vehicle control, are indicated below the corresponding blots. (E) MV4-11 cells and THP-1 cells (cultured in glucose or galactose containing media) were treated with VEN, ONC, or ISRIB, alone or in combination, for 24 h and then stained with annexin V/PI and analyzed via flow cytometry. * p < 0.05, *** p < 0.001, and ns not significant compared to the same drug treatment but without ISRIB