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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Phenotypic diversity of CTCs and tdEVs in liquid biopsies of tumour-draining veins is linked to poor prognosis in colorectal cancer

Fig. 1

Identification of CTCs and tdEVs with the CellSearch system. (A) Representative circulating tumour cells (CTCs), tumour-derived extracellular vesicles (tdEVs), white blood cells (WBCs), lymphocyte-derived extracellular vesicles (ldEVs) and bare nuclei identified using the ACCEPT tool. For every object, a thumbnail image overlay of the three fluorescent channels (CD45, DAPI, PE) and each channel separately is shown. The red contour indicates the detected boundary of each object. The scale bar represents 10 pixels equivalent to 6.4 μm. (B) UMAP visualization of the different types of events identified with ACCEPT in samples from CRC R0 patients from the combined DU + HE cohort (N = 93 CVCs; N = 134 DVs). All detected CTCs (N = 5870) are represented, while for purposes of better visualization, for all other populations, we randomly selected N = 5870 objects. (C) Heatmap representation of chromosomal copy number alterations (CNAs) of 20 single CTCs obtained by low-pass NGS after single-cell whole-genome amplification. (D) Detection rates (positivity rates) and counts of CTCs and (E) tdEVs in samples from CRC R0 patients from the combined DU + HE cohort (N = 93 CVCs; N = 134 DVs). The horizontal lines represent the median. *p < 0.05

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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