Fig. 2

Effects of lurbinectedin, ecubectedin and PM54 on DNA damage and repair machinery in MPM cells. (A) The expression pattern of DNA damage genes up-regulated (blue) and down-regulated (red) in MPM cell lines after a 24 h treatment with cisplatin + pemetrexed (Pt + PMX), lurbinectedin (L), ecubectedin and PM54 at their IC₅₀ for 24 h, was shown as mRNA abundance versus a pool of housekeeping genes (n = 3 independent experiments, in triplicates). (B) Histograms showing the percentage of total DNA in the tail of Comet assay in MPM cells treated with Pt + PMX, L, ecubectedin and PM54 at IC₅₀ for 24 h. Data are expressed as means ± SD of 12 MPM samples (n = 3 independent experiments, in duplicates). *p < 0.05, ***p < 0.001: vs. untreated cells; °°°p < 0.001: vs. Pt + PMX. (C) Representative Comet assay images of MPM#1 (epithelioid, Bap positive: Epi, BAP+) and MPM#7 (sarcomatoid, BAP1 negative: Sar, BAP-)