Fig. 1

Antiproliferative and anti-invasive effects of lurbinectedin, ecubectedin and PM54 in patient-derived MPM cells. (A) In vitro IC₅₀ and IC₁₀ median values of cisplatin plus pemetrexed (Pt + PMX), lurbinectedin, ecubectedin and PM54 determined in 12 patient-derived histotypes of MPM (epithelioid: square; sarcomatoid: circle; biphasic: diamond) with BAP1 positive (solid symbol) and BAP1 negative (open symbol). Results are mean of 4 independent experiments. (B) Representative images of crystal violet staining obtained on long-term (10 weeks) assay with APN#7 cells (sarcomatoid, BAP1-). (C). Representative images of the effect induced by the compounds to six different MPM spheroids following 72 h of incubation. Scale bar = 100 μm. (D). Representative phase images of migrated cells (scale bar, 100 μm) and (E) cells in the lower chamber of APN#1 (epithelioid, BAP1+) and APN#7 (sarcomatoid, BAP1-)