Fig. 3
From: Targeting PRMT5 through PROTAC for the treatment of triple-negative breast cancer
YZ-836P induces cell cycle arrest and promotes apoptosis in TNBC. (A) YZ-836P increased the proportion of cells in the G1 phase. HCC1806 and HCC1937 cells were incubated with YZ-836P, stained with PI, and analyzed using flow cytometry (48 h). (B) YZ-836P induced the G1 phase cell cycle arrest in both cell lines. (C) YZ-836P regulated the expression levels of cell cycle-related proteins, including Cyclin D1, CDK6, CDK4, p21, and p27, as detected by WB. (D) YZ-836P induces apoptosis in both cell lines, as measured by Annexin V-PI double staining and flow cytometry. (E) Statistical results of panel D. (F) YZ-836P regulated the expression of apoptosis-related proteins, including cleaved Caspase 3 and PARP, XIAP, and Mcl-1, as detected by WB. Data represent results from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001; ns, not significant