Fig. 4

S1PR1 regulates COL5A1, MMP1 and SERPINE1 through the transcription factor FOXA1. A Potential transcription factors that regulates the expression of COL5A1, MMP1, and SERPINE1 in the hTFtarget database. B Heatmap of the transcription factors in RNA sequencing data. C mRNA levels of FOXA1 and CEBPB detected by RT-qPCR in A549 and H1975 cells with S1PR1 overexpression. D Protein levels of FOXA1 and CEBPB detected by western blot in A549 and H1975 cells with S1PR1 overexpression. E Protein levels of FOXA1 detected by western blot in H1975 cells with S1PR1knockdown. F The motif of FOXA1 predicted by JASPAR database. G The potential binding sites of FOXA1 in promoters of COL5A1, MMP1 and SERPINE1 were predicted by JASPAR database. H ChIP-qPCR of FOXA1 binding to promoters of COL5A1, MMP1 and SERPINE1 in A549 cells. I ChIP-qPCR of FOXA1 binding to promoters(P1) of COL5A1, MMP1 and SERPINE1 in A549 and H1975 cells with S1PR1 overexpression. J Predicted binding sites of FOXA1 in promoters of COL5A1, MMP1 and SERPINE1 and matched mutant sequences. H Luciferase reporter assay showed the luciferase activity of FOXA1 on the wildtype and mutant promoter of COL5A1, MMP1 and SERPINE1. ns, not significant, *P < 0.05, **P < 0.01 and ***P < 0.001