Fig. 3
From: S1PR1 suppresses lung adenocarcinoma progression through p-STAT1/miR-30c-5 p/FOXA1 pathway
S1PR1 inhibits the malignant function of LUAD by regulating extracellular matrix through COL5A1, MMP1, SERPINE1. A Volcano plot and B heatmap displayed the DEGs between A549 cells with NC or OE-S1PR1. C t-SNE analysis of the DEGs between A549 cells with NC or OE-S1PR1. D GO function and KEGG pathway enrichment analysis of the DEGs. E GSEA enrichment analysis of KEGG pathway between A549 cells with NC or OE-S1PR1. F PPI network construction of the DEGs between A549 cells with NC or OE-S1PR1. H Hub genes screened using cytoHubba plugin in cytoscape. H mRNA expression levels of selected hub genes detected by RT-qPCR in A549 and H1975 cells with S1PR1 overexpression. I protein levels of COL5A1, MMP1, and SERPINE1 detected by western blot in A549 and H1975 cells with S1PR1 overexpression. J mRNA expression levels of COL5A1, MMP1, and SERPINE1 detected by RT-qPCR in H1975 cells with S1PR1 knockdown. (K) Protein levels of COL5A1, MMP1, and SERPINE1 detected by western blot in H1975 cells with S1PR1 knockdown. ns, not significant, *P < 0.05, **P < 0.01 and ***P < 0.001