Fig. 1

hCAFs promoted the migration and viability of iCCA cells in co-culture conditions. (A). Schematic representation of the experimental model of KKU-M213 and KKU-M156 migration without and with hCAFs. (B). Representative images of KKU-M213 (panels on the left) and KKU-M156 cells (panels on the right) migrated alone or in co-culture with three hCAFs (#1, #2, #3) using the transwell migration system. The migration of both iCCA cell lines was significantly increased after co-culture with hCAFs. Cells that migrated were counted in five random microscope fields for each sample (10× magnification, scale bar 100 μm). (C). The KKU-M213 and KKU-M156 cell viability was evaluated by CyQUANT™ XTT Cell Viability kit. The viability of both iCCA cells was significantly increased in co-culture with hCAFs. (D). The KKU-M213 and KKU-M156 cell proliferation was evaluated by Trypan Blue cell counting. Numbers of both iCCA cells were significantly increased in co-culture with hCAFs. Data are expressed as the mean ± SD of three independent experiments with three hCAFs. *p < 0.05 **p < 0.01 and ***p < 0.001